Which of the following is the correct sequence that describes the excitation and contraction of a skeletal muscle fiber? 1. Tropomyosin shifts and unblocks the cross-bridge binding sites. 2. Calcium is released and binds to the troponin complex. 3. Transverse tubules depolarize the sarcoplasmic reticulum. 4. The thin filaments are ratcheted across the thick filaments by the heads of the myosin molecules using energy from ATP. 5. An action potential in a motor neuron causes the axon to release acetylcholine, which depolarizes the muscle cell membrane.